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DnaA · P03004

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Meet the protein

DnaA is UniProt protein record P03004 for Escherichia coli (strain K12) (NCBI 83333). The retained sequence contains 467 amino acids.

[1]
Evidence for this statement

Source annotation

What is known about function

Function and evidence from UniProt

Inspect structured data
source text
Plays an essential in the initiation and regulation of chromosomal replication. Binds in an ATP-dependent fashion to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle (PubMed:3036372). Binds the DnaA box (consensus sequence 5'-TTATC[CA]A[CA]A-3') and separates the double-stranded (ds)DNA (PubMed:3036372, PubMed:18216012). Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior (By similarity). The ATP-DnaA-oriC complex binds and stabilizes the upper strand of the AT-rich DNA unwinding element (DUE) (PubMed:18216012). Mutagenesis of residues that line the central pore blocks dsDNA strand separation (PubMed:18216012). Subsequent binding of DNA polymerase III subunits leads to replisome formation (PubMed:3036372, PubMed:18216012). The DnaA-ATP form converts to DnaA-ADP; once converted to ADP the protein cannot initiate replication, ensuring only 1 round of replication per cell cycle (PubMed:3036372). Binds ATP, ADP and dATP equally well, hydrolyzes ATP with a half-life of about 15 minutes, ATP hydrolysis is not required for pre-priming replisome formation, nucleotide exchange is very slow (PubMed:3036372). Binds acidic phospholipids (PubMed:9478970, PubMed:26272946). DnaA inhibits its own gene expression (PubMed:2981626) as well as that of other genes including dam, rpoH (PubMed:2540187), ftsA and mioC
source language
en
evidence codes
  • evidenceCode
    ECO:0000250
    source
    UniProtKB
    id
    O66659
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    18216012
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    2540187
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    26272946
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    2981626
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    3036372
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    9478970
[1]
Evidence for this statement

Source annotation

Evidence codes qualify the annotation. This record is not a measurement of an illustrated specimen.

Function and evidence from UniProt

Inspect structured data
source text
Also required for replication of some plasmid DNA; binds 4 DnaA boxes in the minimal plasmid RK2 replication origin (oriV)
source language
en
evidence codes
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    15878847
  • evidenceCode
    ECO:0000269
    source
    PubMed
    id
    9242693
[1]
Evidence for this statement

Source annotation

Evidence codes qualify the annotation. This record is not a measurement of an illustrated specimen.

Sequence and version

P03004

Inspect structured data
accession
P03004
name
Chromosomal replication initiator protein DnaA
length
467
entry version
211
sequence version
2
sequence
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
GLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNV
PAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTH
LLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELE
TRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVR
EALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNH
SLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
[1]
Evidence for this statement

Source annotation

Genes and domains

Cross-references supplied by UniProt

Inspect structured data
gene symbols
  • dnaA
gene crossreferences
  • database
    GeneID
    id
    93778443
    properties
    • key
      Description
      value
      -
  • database
    GeneID
    id
    948217
    properties
    • key
      Description
      value
      -
interpro domains
  • provider
    interpro
    accession
    IPR003593
    name
    AAA+_ATPase
  • provider
    interpro
    accession
    IPR001957
    name
    Chromosome_initiator_DnaA
  • provider
    interpro
    accession
    IPR020591
    name
    Chromosome_initiator_DnaA-like
  • provider
    interpro
    accession
    IPR018312
    name
    Chromosome_initiator_DnaA_CS
  • provider
    interpro
    accession
    IPR013159
    name
    DnaA_C
  • provider
    interpro
    accession
    IPR013317
    name
    DnaA_dom
  • provider
    interpro
    accession
    IPR024633
    name
    DnaA_N_dom
  • provider
    interpro
    accession
    IPR038454
    name
    DnaA_N_sf
  • provider
    interpro
    accession
    IPR027417
    name
    P-loop_NTPase
  • provider
    interpro
    accession
    IPR010921
    name
    Trp_repressor/repl_initiator
[1]
Evidence for this statement

Source annotation

A database cross-reference does not replace assembly and strain checks. P03004 belongs to K12 (83333); the prior gene profile belongs to MG1655 (511145). These taxa are not merged.

GO function annotations

A sample with evidence codes

Inspect structured data

Shown / materialized / in provider response: 12 / 54 / 54

GO
GOEvidencePublication or ruleAssigned by
GO:0006260
DNA replication
Biological Process		ECO:0000315
acts_upstream_of_or_within		PMID:12068813EcoliWiki
20100503
GO:0006275
regulation of DNA replication
Biological Process		ECO:0000315
acts_upstream_of_or_within		PMID:12068813EcoliWiki
20100503
GO:0003677
DNA binding
Molecular Function		ECO:0000256
enables		GO_REF:0000002InterPro
20260727
GO:0003677
DNA binding
Molecular Function		ECO:0000314
enables		PMID:7615570EcoCyc
20110629
GO:0003688
DNA replication origin binding
Molecular Function		ECO:0000318
enables		GO_REF:0000033GO_Central
20260424
GO:0003688
DNA replication origin binding
Molecular Function		ECO:0000501
enables		GO_REF:0000120UniProt
20260727
GO:0003688
DNA replication origin binding
Molecular Function		ECO:0000315
enables		PMID:19833870EcoCyc
20110629
GO:0005515
protein binding
Molecular Function		ECO:0000353
enables		PMID:10972842IntAct
20260725
GO:0005515
protein binding
Molecular Function		ECO:0000353
enables		PMID:15690043IntAct
20260725
GO:0005515
protein binding
Molecular Function		ECO:0000353
enables		PMID:15690043IntAct
20260725
GO:0005515
protein binding
Molecular Function		ECO:0000353
enables		PMID:17699754IntAct
20260725
GO:0005515
protein binding
Molecular Function		ECO:0000353
enables		PMID:18179598IntAct
20260725
[2]
Evidence for this statement

Source annotation

These are source annotations, not independent proof of a causal mechanism. The complete captured responses are available as originals.

Research cited by the protein record

Bibliographic records

Inspect structured data
  • source
    uniprot
    id
    P03004-citation-6296774
    title
    The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
    pmid
    6296774
    doi
    10.1093/nar/10.22.7373
    year
    1982
    authors
    • Hansen E.B.
    • Hansen F.G.
    • von Meyenburg K.
    journal
    Nucleic Acids Res.
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-6234204
    title
    Structural analysis of the dnaA and dnaN genes of Escherichia coli.
    pmid
    6234204
    doi
    10.1016/0378-1119(84)90253-1
    year
    1984
    authors
    • Ohmori H.
    • Kimura M.
    • Nagata T.
    • Sakakibara Y.
    journal
    Gene
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-7686882
    title
    DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.
    pmid
    7686882
    doi
    10.1006/geno.1993.1230
    year
    1993
    authors
    • Burland V.D.
    • Plunkett G. III
    • Daniels D.L.
    • Blattner F.R.
    journal
    Genomics
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-9278503
    title
    The complete genome sequence of Escherichia coli K-12.
    pmid
    9278503
    doi
    10.1126/science.277.5331.1453
    year
    1997
    authors
    • Blattner F.R.
    • Plunkett G. III
    • Bloch C.A.
    • Perna N.T.
    • Burland V.
    • Riley M.
    • Collado-Vides J.
    • Glasner J.D.
    • Rode C.K.
    • Mayhew G.F.
    • Gregor J.
    • Davis N.W.
    • Kirkpatrick H.A.
    • Goeden M.A.
    • Rose D.J.
    • Mau B.
    • Shao Y.
    journal
    Science
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-16738553
    title
    Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
    pmid
    16738553
    doi
    10.1038/msb4100049
    year
    2006
    authors
    • Hayashi K.
    • Morooka N.
    • Yamamoto Y.
    • Fujita K.
    • Isono K.
    • Choi S.
    • Ohtsubo E.
    • Baba T.
    • Wanner B.L.
    • Mori H.
    • Horiuchi T.
    journal
    Mol. Syst. Biol.
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-6329723
    title
    The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
    pmid
    6329723
    doi
    10.1002/j.1460-2075.1982.tb01294.x
    year
    1982
    authors
    • Hansen F.G.
    • Hansen E.B.
    • Atlung T.
    journal
    EMBO J.
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-2981626
    title
    Autoregulation of the DNA replication gene dnaA in E. coli K-12.
    pmid
    2981626
    doi
    10.1016/0092-8674(85)90319-8
    year
    1985
    authors
    • Braun R.E.
    • O'Day K.
    • Wright A.
    journal
    Cell
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-3036372
    title
    ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome.
    pmid
    3036372
    doi
    10.1016/0092-8674(87)90221-2
    year
    1987
    authors
    • Sekimizu K.
    • Bramhill D.
    • Kornberg A.
    journal
    Cell
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-2540187
    title
    dnaA protein regulates transcriptions of the rpoH gene of Escherichia coli.
    pmid
    2540187
    doi
    10.1016/s0021-9258(18)83238-0
    year
    1989
    authors
    • Wang Q.P.
    • Kaguni J.M.
    journal
    J. Biol. Chem.
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-9478970
    title
    Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer.
    pmid
    9478970
    doi
    10.1074/jbc.273.9.5167
    year
    1998
    authors
    • Garner J.
    • Durrer P.
    • Kitchen J.
    • Brunner J.
    • Crooke E.
    journal
    J. Biol. Chem.
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-9674428
    title
    The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E. coli chromosomal replicase.
    pmid
    9674428
    doi
    10.1016/s0092-8674(00)81222-2
    year
    1998
    authors
    • Katayama T.
    • Kubota T.
    • Kurokawa K.
    • Crooke E.
    • Sekimizu K.
    journal
    Cell
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
  • source
    uniprot
    id
    P03004-citation-9298644
    title
    Escherichia coli proteome analysis using the gene-protein database.
    pmid
    9298644
    doi
    10.1002/elps.1150180805
    year
    1997
    authors
    • VanBogelen R.A.
    • Abshire K.Z.
    • Moldover B.
    • Olson E.R.
    • Neidhardt F.C.
    journal
    Electrophoresis
    rights reuse state
    metadata_only
    source url
    https://rest.uniprot.org/uniprotkb/P03004.json
    source snapshot sha256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
[1]
Evidence for this statement

Source annotation

Only metadata is shown. Article text has a separate reuse decision.

Organism in the UniProt record

Escherichia coli (strain K12) · NCBI 83333Scientific profile →

Your research question

Save the article to return to your question and this source version.

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Sources and versions

An explanation grounded in the cited sources

  1. UniProt Consortium · P03004

    2026-09-09T03:19:40.940804+00:00 · CC-BY-4.0

    Provenance and original
    Source version
    entry 211; sequence 2
    Original SHA-256
    20b9ed0385942a0aebc3645f0f499a77e9aab0741218e2327ee80b6ae7590dca
    Processing
    Unmodified provider response; selected fields are projected into the document.
    Version details
    Native entry and sequence versions retained.

    View original JSON · Download original

  2. Gene Ontology Consortium / QuickGO · UniProtKB:P03004

    2026-09-09T03:20:28.660556+00:00 · CC-BY-4.0

    Provenance and original
    Original SHA-256
    30a929f57c0f50a1fe5ae1509981b7f62b7e077f02ef20ea6320183d3865f8ac
    Processing
    Unmodified provider response; selected fields are projected into the document.
    Version details
    No native GO release was supplied; exact response hash and retrieval time are retained.

    View original JSON · Download original

Inspect structured data
Content version
4ca9013788258528077a3a10283bf04535988685b887158aa302eba857fc1177
Source version
research-20260909.1
Updated
2026-09-09